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NHLBI Proteomics Administrative and Coordination Center

Investigator's Corner

Question and Answer Session:
Q. Which is your favorite Mass spec? Do you have good services with Thermo-Fisher? We have many problems with the LTQ configurations, programs, updates..etc.and very little support in the island.
Submitted By: Loyda M Melendez, PhD - Affiliation: University of Puerto Rico Medical Sciences Campus Key Proteomics Director

A. That is an excellent question and I have asked Dr. Sue Weintraub, the mass spectrometry director for the San Antonio Cardiovascular Proteomics Center, to help me with the answer. Here is her answer: I have used Thermo Fisher mass spectrometers for many years (starting in 1971 when the company was Finnigan) and I have been completely satisfied with both the operation of the instruments and the service provided by the company. We currently use an LTQ and an LTQ Orbitrap Velos for protein identification and quantification. The choice of which instrument to use depends on the questions being asked in the experiment. The LTQ is highly satisfactory for protein identification of simple and complex mixtures. We try to reserve the Orbitrap for quantitative analysis (label-free and various types of stable isotope labeling), for locating sites of modification, and for samples that are of low abundance. Are there just not enough service engineers in Puerto Rico or are you having unusual problems with your instrument? I will be happy to talk to you about your instrument problems if youÂ’d like. Please let me know the best way to contact you (Weintraub@uthscsa.edu).
Last Updated: 11-15-11
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